Duvenage, who played 98 times and captained Italian side Benetton regularly, also has 72 caps for French side Perpignan under his belt, and his experience since returning to Cape Town has been invaluable for the Stormers.
His milestone won’t be the only one on Friday night - lock Adre Smith gets his 50th cap for the Stormers as well.
Duvenage is the only change to the backline that beat defending champions Leinster 35-0 in the opening game of the competition, while there are two changes to the forward pack - both in the loose trio.
Director of Rugby John Dobson paid tribute to the two milestone achievers.
“Dewaldt comes from the generation of players that gave us the likes of Brok Harris and Deon Fourie and it is fitting that he joins them as a DHL Stormers centurion. He has always been very tactically astute and there are few others as committed to the cause.
“Adré has come back from his time in Japan a better player and while this is a significant achievement, it is almost more exciting to see where he can take his game in the future,” he added.
Evan Roos heads up the back row from No 8, with hard-working Marcel Theunissen and Connor Evans joining him in a revamped back row. Last weekend’s loose forwards - Paul de Villiers and Ben-Jason Dixon both are on the bench for a second half impact for the side.
That means Wandisile Simelane will continue at 15 for the side in a role he didn’t relish when he was playing for the Bulls.
Front row forwards Ali Vermaak and Sazi Sandi will both make their first appearance of the season in the only other changes on the replacements bench.
Stormers Director of Rugby John Dobson believes the team needs to use the momentum they gained in the win over Leinster and get another result at home.
“While we were happy with the result last week, we know that there can be no complacency heading into this match against an Ospreys side that have proven tough customers for us in the past.
“There are a few areas we want to improve on and while we have maintained the core of the team that did duty last week, we are also looking forward to seeing what the players coming in can add,” he said.
DHL Stormers: Wandisile Simelane; Seabelo Senatla, Ruhan Nel (captain), Dan du Plessis, Leolin Zas; Jurie Matthee, Dewaldt Duvenage; Evan Roos, Conor Evans, Marcel Theunissen, JD Schickerling, Adre Smith, Neethling Fouche, Andre-Hugo Venter, Vernon Matongo.
Replacements: JJ Kotze, Ali Vermaak, Sazi Sandi, Ruben van Heerden, Ben-Jason Dixon, Paul de Villiers, Stefan Ungerer, Clinton Swart