Noah Lyles and Sha'Carri Richardson, fresh off 100-metre titles at the World Athletics Championships, will run the event in next week's Prefontaine Classic, the Diamond League Final.
Meet organisers announced the US sprint stars would compete at the September 16 showdown at Hayward Field in Eugene, Oregon.
Richardson followed her 100m victory at the US championships in Eugene in July by winning the 100 world crown and taking third at 200m last month in Budapest as well as helping the US women's 4x100 relay team capture gold.
Lyles staked his claim as the world's fastest man by sweeping the 100 and 200 world titles and helping a US 4x100 men's relay team triumph.
Lyles is a four-time Diamond League Final champion in the 200, taking the crown in 2017, 2018, 2019 and 2022. He seeks his first title at 100m after setting a wind-legal personal best of 9.83 seconds this year to rank joint first in the world.