Roma blew their chance to move into the top three in Serie A when they slipped to a 1-0 defeat at Udinese on Monday.
Victory would have lifted Gian Piero Gasperini's side from fifth to third, level on points with Napoli and just one point behind second-placed AC Milan who face Bologna on Tuesday.
However, a lone goal from Jurgen Ekkelenkamp four minutes into the second half was enough to consign them to defeat and leave them a point behind Juventus who moved into the fourth Champions League spot with victory over Parma on Sunday.
The win lifts Udinese to ninth, still nine points short of the European places.
Inter Milan are the runaway leaders of Serie A, extending their lead at the top to eight points on Sunday with a 2-0 win at Cremonese.