Uncapped quartet Noni Madueke, Morgan Gibbs-White, Tino Livramento and Angel Gomes were named in England interim coach Lee Carsley's first squad on Thursday.
Carsley has been appointed on a temporary basis for Nations League fixtures against the Republic of Ireland and Finland next month.
Gareth Southgate stepped down in July after England lost the Euro 2024 final to Spain.
Carsley is without injured Real Madrid star Jude Bellingham.
Kieran Trippier announced his retirement from international football earlier on Thursday.
Manchester City's Kyle Walker was left out, alongside other Euro 2024 squad members Ivan Toney, Aaron Ramsdale, Joe Gomez, Lewis Dunk and Adam Wharton.
FULL SQUAD
Goalkeepers: Jordan Pickford (Everton), Nick Pope (Newcastle), Dean Henderson (Crystal Palace)
Defenders: John Stones, Rico Lewis (both Manchester City), Harry Maguire (Manchester United), Marc Guehi (Crystal Palace), Ezri Konsa (Aston Villa), Levi Colwill (Chelsea), Tino Livramento (Newcastle), Trent Alexander-Arnold (Liverpool)
Midfielders: Declan Rice (Arsenal), Kobbie Mainoo (Manchester United), Conor Gallagher (Atletico Madrid/ESP), Angel Gomes (Lille/FRA), Phil Foden (Manchester City), Morgan Gibbs-White (Nottingham Forest), Cole Palmer (Chelsea)
Forwards: Harry Kane (Bayern Munich/GER), Ollie Watkins (Aston Villa), Jarrod Bowen (West Ham), Bukayo Saka (Arsenal), Anthony Gordon (Newcastle), Jack Grealish (Manchester City), Eberechi Eze (Crystal Palace), Noni Madueke (Chelsea)