Ibrahima Konate's five-year stay at Liverpool will end when his contract expires next month after failing to agree a new deal, the club confirmed on Sunday.
The France international said last month he was confident of agreeing terms with the Premier League giants.
However, no breakthrough in negotiations was found and he will become the third high profile player this season to walk away from Anfield on a free transfer.
Mohamed Salah and Andy Robertson have also departed after a glittering nine years on Merseyside.
"Liverpool FC can confirm Ibrahima Konate is set to leave the club upon the expiry of his contract this summer," the club said in a statement.
"Konate will depart with our gratitude and appreciation for the contribution he made and everyone at the club wishes him the best for the future."
Konate was a major part of the Liverpool side that stormed to the Premier League title in the 2024/25 season.
He also won two League Cups and the FA Cup during his time at Anfield.
But the 27-year-old's form dipped drastically at the beginning of this season during a difficult campaign for the Reds.
It was also a season marked by tragedy for Konate, whose father died earlier this year, and for the club after the loss of forward Diogo Jota in a car accident.
"Representing this club has been an honour. We've shared incredible moments together… highs and lows, trophies, challenges, lifelong friendships, and heartbreaking moments that will stay with us forever, none more painful than losing our brother Diogo," Konate said in a statement.
"Losing my father this year was one of the hardest periods of my life, but even through hardship, my commitment to this club never changed. During the toughest moments, I gave everything I had for this badge."
Arne Slot was sacked on Saturday after finishing fifth in the Premier League, 25 points behind champions Arsenal.
Paris-born Konate has been linked with a move to European champions Paris Saint-Germain.
His departure could force Liverpool into the transfer market for more defensive cover despite the arrival next season of Jeremy Jacquet in a £60 million ($81 million) move from Rennes.