Alvaro Bautista, the reigning World Superbike champion, secured a double victory at Assen, winning the Superpole Race after outpacing Jonathan Rea.
The result means he will start Race 2 from the front of the grid, having already won Race 1 on Saturday.
Bautista had been penalised with a three-place grid penalty for Race 1 alongside Axel Bassani due to slow riding during qualifying, but he once again started the Superpole Race in first position.
Before the race began, Loris Baz encountered a mechanical problem during the formation lap and stopped at the side of the track. Liquid from Baz's M 1000 RR at turn seven delayed the race start, and the organisers confirmed a new schedule of 8 laps after a 15-minute delay.
Rea had a fantastic launch at the start of the race and took the lead, with Bautista following closely behind. However, Bautista regained the lead before the end of the first lap, with Razgatlioglu and Lowes close behind.
Despite appearing to be pushing his Ducati to the limit and sliding on a few occasions, Bautista could not pull clear of Rea, Razgatlioglu, and Lowes.
With three laps remaining, Rea attempted to make a move on Bautista on the run to turn seven, but he was unsuccessful.
Bautista then set the fastest lap of the race with two laps remaining, before opening up a gap and securing the victory.
After the top four racers, Andrea Locatelli lead the second group after a late move on Bassani, who managed to keep his position in sixth place after overtaking Dominique Aegerter on the last lap.
SUPERPOLE RACE RESULTS
1. Alvaro Bautista SPA Aruba.It Racing Ducati 8 Laps
2. Jonathan Rea GBR Kawasaki Racing Team WorldSBK +0.916s
3. Toprak Razgatlioglu TUR Pata Yamaha Prometeon WorldSBK +1.757s
4. Alex Lowes GBR Kawasaki Racing Team WorldSBK +3.126s
5. Andrea Locatelli ITA Pata Yamaha Prometeon WorldSBK +6.067s
6. Axel Bassani ITA Motocorsa Ducati +6.781s
7. Dominique Aegerter SWI GYTR GRT Yamaha WorldSBK Team +7.054s
8. Scott Redding GBR ROKIT BMW Motorrad WorldSBK Team +7.125s
9. Xavi Vierge SPA Team HRC WorldSBK +8.568s
10. Michael Van Der Mark NED ROKIT BMW Motorrad WorldSBK Team +10.344s
11. Iker Lecuona SPA Team HRC WorldSBK +10.546s
12. Remy Gardner AUS GYTR GRT Yamaha WorldSBK Team +11.246s
13. Michael Ruben Rinaldi ITA Aruba.It Racing Ducati +11.807s
14. Philipp Oettl GER GoEleven Ducati +12.037s
15. Danilo Petrucci ITA Barni Spark Racing Team +12.825s
16. Bradley Ray GRB Motoxracing Yamaha WorldSBK Team +13.079s
17. Garrett Gerloff USA Bonovo Action BMW +13.549s
18. Hafizh Syahrin MAL PETRONAS MIE Racing HONDA Racing +20.193s
19. Tom Sykes GBR Puccetti Kawasaki +21.340s
20. Lorenzo Baldassarri ITA GMT94 Yamaha +24.463s
21. Eric Granado BRA PETRONAS MIE Racing HONDA Racing +25.353s
22. Gabriele Ruiu ITA Bmax Racing +25.706s
23. Oliver Konig CZE Orelac Racing Kawasaki +27.243s
24. Isaac Vinales SPA TPR by Vinales Racing DNF
25. Loris Baz FRA Bonovo Action BMW DNS