Scrum-half Tomos Williams has been released from the Wales squad for the remaining November internationals due to a shoulder injury, with Kieran Hardy replacing him.
The Gloucester No 9 was hurt during Wales' 24-19 defeat against Fiji earlier this month.
He had already been ruled out of Sunday's match with Australia, being replaced in the starting line-up by Ellis Bevan, with Rhodri Williams gaining a place on the bench.
Tomos Williams will also not be available to face world champions South Africa in Cardiff on November 23.
Experienced Ospreys scrum-half Hardy has been called up to the squad as replacement for Williams, the Welsh Rugby Union said.
Defeat against Australia would be a record 11th successive test loss for Wales.