The Australian Open was hit by two more withdrawals on Saturday with 11th seed Paula Badosa and home hope Ajla Tomljanovic both injured.
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The first Grand Slam of the year has seen several players pull out ahead of the opening day on Monday, including the injured men's No 1 Carlos Alcaraz and Naomi Osaka, who is pregnant.
Spain's former world No 2 Badosa became the latest high-profile casualty because of a thigh injury picked up this week in a warm-up tournament.
"Bad news... during the quarterfinal match in Adelaide I hurt myself and after receiving the results I will have to be out for a few weeks," the 25-year-old tweeted.
"Sad to miss Australian Open this year, really going to miss it.
"I will do everything I can to return as soon as possible."
Also ruled out on Saturday was Australia's 35th-ranked Tomljanovic because of a knee injury.
They will be replaced by Brazil's Laura Pigossi and Leolia Jeanjean of France.