Spain coach Luis de la Fuente called up Real Betis midfielder Isco on Monday for the Nations League final four, six years after his last appearance for La Roja.
The 33-year-old has enjoyed a superb season with Betis, helping them reach the Conference League final where they will face Chelsea on Wednesday.
Euro 2024 winners Spain face France in the semifinal in Stuttgart on 5 June before a potential final against Germany or Portugal.
Real Madrid's new defensive signing from Bournemouth, Dean Huijsen, who impressed in the quarterfinal win over the Netherlands, returns to the squad, while Barcelona midfielders Gavi and Fermin Lopez also make the cut.
Five world champions and a call-up to perpetuate our European throne.
— Spanish Football (@SpainIsFootball) May 26, 2025
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Teenage winger Lamine Yamal and playmaker Pedri are the key figures, along with Athletic Bilbao attacker Nico Williams.
Manchester City holding midfielder Rodri was not included despite his recent return to fitness, with Real Sociedad's Martin Zubimendi set to start at the base of midfield.
Full Spain squad for Nations League final four:
Goalkeepers: Unai Simon (Athletic Bilbao), David Raya (Arsenal), Alex Remiro (Real Sociedad)
Defenders: Oscar Mingueza (Celta Vigo), Pedro Porro (Tottenham), Dani Vivian (Athletic Bilbao), Pau Cubarsi (Barcelona), Robin Le Normand (Atletico Madrid), Dean Huijsen (Real Madrid), Alejandro Grimaldo (Bayer Leverkusen), Marc Cucurella (Chelsea)
Midfielders: Martin Zubimendi (Real Sociedad), Gavi, Fermin Lopez, Pedri Gonzalez, Dani Olmo (all Barcelona), Isco (Real Betis), Mikel Merino (Arsenal), Fabian Ruiz (PSG), Alex Baena (Villarreal)
Forwards: Lamine Yamal (Barcelona), Nico Williams (Athletic Bilbao), Yeremy Pino (Villarreal), Mikel Oyarzabal (Real Sociedad), Samu Omorodion (Porto), Alvaro Morata (Galatasaray)